TGE FT-ICR


Partenaires

CNRS
Logo Ecole Polytechnique
Logo UPS Logo UDL
Logo Sorbonne Universite Logo Universite de Lille 1
Logo Rouen Logo INSA Rouen Normandie



Accueil du site > Production scientifique > IRMPD Spectroscopy : Evidence of Hydrogen Bonding in the Gas Phase Conformations of Lasso Peptides and their Branched-Cyclic Topoisomers

IRMPD Spectroscopy : Evidence of Hydrogen Bonding in the Gas Phase Conformations of Lasso Peptides and their Branched-Cyclic Topoisomers

Date de publication: 2 juin 2016

Jeanne Dit Fouque, Kevin ; Lavanant, Hélène ; Zirah, Séverine ; Steinmetz, Vincent ; Rebuffat, Sylvie ; Maître, Philippe ; Afonso, Carlos
J. Phys. Chem. A 120 3810-3816 (2016). DOI

Travail réalisé sur le site de l’Université Paris Sud.

Abstract

Lasso peptides are natural products characterized by a mechanically interlocked topology. The conformation of lasso peptides has been probed in the gas phase using ion mobility-mass spectrometry (IM-MS) which showed differences in the lasso and their unthreaded branched-cyclic topoisomers depending on the ion charge states. To further characterize the evolution of gas phase conformations as a function of the charge state and to assess associated changes in the hydrogen bond network, infrared multiple photon dissociation (IRMPD) action spectroscopy was carried out on two representative lasso peptides, microcin J25 (MccJ25) and capistruin, and their branched-cyclic topoisomers. For the branched-cyclic topoisomers, spectroscopic evidence of a disruption of neutral hydrogen bonds were found when comparing the 3+ and 4+ charge states. In contrast, for the lasso peptides, the IRMPD spectra were found to be similar for the two charge states, suggesting very little difference in gas phase conformations upon addition of a proton. The IRMPD data were thus found consistent and complementary to IM-MS, confirming the stable and compact structure of lasso peptides in the gas phase.